This is really a step above even your usually-high level of quality. Seriously, this is very cool.
Now since it's not being said elsewhere I may as well try to give a couple comments. I've said it before and I'll likely never stop, but this could be SO much more if you gave it a good careful refining. Parts that could use it most are the wobbly edges of the yellow in the background flame, the bizarre bulging width near the end of the tail, and the mishmash of scales that are close to us. The black outline of the dragon as a whole could use some smoothing too.
There's an unfortunate circumstance of color theory happening here. The red on the outside is such a strong bold color compared to the neutral blues/greys/purples/yellows that the background is kinda swallowing up the dragon himself. Okay, I checked the color count to see if you had spare room to maybe make a new darker/less-intense red for the background, and found that you have 7 colors you're primarily using but have a whole 14 extra shades only barely off the base colors, mostly centered around the lower head. Here, this is their locations: [link] I recognize the phenomenon that causes the color weirdness over on the yellow. Any chance you were playing around with partial transparency at all and drawing over it? Because that particular color differential is pretty common to that. Alternatively, if you were using brushes that had a little auto-AA on them while sketching things out that could cause it. While I'm harping on the bright red in the background eating everything up, as far as colors go on their own this is a freakin' gorgeous palette.
Here's a pretty good question: Where's the lightsource? Underbelly scales and mane say upper-left, as does the face flesh. Scales on the tail say a variety of directions (from the left, from the right, from above, from in front of the face), and the horns say from the left. The spikes along the body say right above the dragon, and the claws on the hands say right beneath them. All this on a bright red and yellow fire background that casts no lights on the dragon itself (that's a design choice and not a lightsource one, just thought I'd mention it). Now, maybe you've got a lightsource in mind here and I'm being dense, but it looks like light is everywhere and nowhere.
Lastly, you may want to consider harshing up the black outline even more when differentiating parts. Especially from a 1x view (rather than the 2x view here), things like the hands are hard to tell from the body, and the effect of the very strong background is magnified. Similarity of colors on both sides of an outline makes the outline even more important or you risk losing the distinction and it all blending together.
All that is me nit-picking to try to give you some things to think about for next time. I was serious at the beginning - this is a damn great piece of pixel art and your style is still one of the most distinct I've seen in recent pixel art.
As an aside, got any intention to stream you pixelin' again any time in the future? Watchin' your process was rather educational when you did it a while back.
Well thanks, I do appreciate it and I can see what you're saying, but I'd like to ask a question; I have been having problems with opacity going down slightly at random times recently, which is why all those colors are there. This has never happened to me before and I have no idea how to fix it?
Hmmm...I think if you head to the PJ forums there's one called "Resources and Support." That might work, but I don't think it's particularly active. My best advice would be to try a reinstall (in case a setting somewhere corrupted) and if after that it's still giving you grief to try the official Adobe troubleshooting forums - they'll be faster and more full of experts than most places would be.
Oh yeah, I have no doubt you're reading it, I'm pretty used to that - I always get pretty carried away with the typing. Maybe I should experiment with bullet points or something just for a change of pace...
It does seem pretty dead, hopefully someone'll know the answer there. Your pieces tend to attract a pretty good amount of attention - it helps that people can recognize your stuff from the front page. Try the reinstall though, that usually helps.